Yes, the Assay buffer that comes as one of the kit components (Product MAK165C) can be used to lyse cells. The kit provides 100 mL of Assay buffer, which is more than enough for the assays as well as to use some for sample preparation. MAK165C does not contain a surfactant, so the cells would need to be lysed by physical disruption...either sonication, or using a Dounce homogenizer (or equivalent).
MAK165
Fluorimetric Hydrogen Peroxide Assay Kit
sufficient for 500 fluorometric tests (red fluorescence)
Synonym(s):
Hydrogen Peroxide Quantification Kit
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1 KIT
$405.00
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 500 fluorometric tests (red fluorescence)
detection method
fluorometric
relevant disease(s)
cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders
storage temp.
−20°C
Related Categories
General description
Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
This kit is suitable to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.
Principle
The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. This kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates a red fluorescent product (λex = 540/λem = 590 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Resp. Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can we use Assay buffer MAK165C included in the kit to lyse cells to make cellular extract to measure endogenous H2O2 level.
1 answer-
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What is the limit of detection for H2O2?
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The lower limit of detection for kit MAK165 is 0.06 uM.
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Does MAK165 recognize all oxidized products or specifically hydrogen peroxide?
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MAK165 is used to quantify hydrogen peroxide in solutions and cell extracts. Therefore, the kit is specific for hydrogen peroxide and does not measure other oxidized products.
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Is the red peroxidase substrate in kit MAK165 the chemical 10-acetyl-3,7-dihydroxyphenoxazine (also known as Amplex Red)?
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The red peroxidase substrate in kit MAK165 is Amplex Red, which is the trademark of Invitrogen. The substrate is 10-Acetyl-3,7-dihydroxyphenoxazine and is chemically the same.
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Are black plates with black bottoms suitable for use with the MAK165 kit, even though the Technical Bulletin recommends black plates with clear bottoms?
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A solid black plate or a black wall with a clear bottom plate is suitable for use.
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