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K1644

Sigma-Aldrich

Anti-KTN1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CG-1 antigen, Anti-Kinectin 1 (kinesin receptor)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
conjugate:
unconjugated
application:
IF
IP
WB
clone:
polyclonal
species reactivity:
human
citations:
5
technique(s):
immunoprecipitation (IP): 5-10 μg using lysate of Jurkat cells
indirect immunofluorescence: 2-5 μg/mL using human HeLa cells
western blot: 2-4 μg/mL using whole extract of human Jurkat cells

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~150 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysate of Jurkat cells
indirect immunofluorescence: 2-5 μg/mL using human HeLa cells
western blot: 2-4 μg/mL using whole extract of human Jurkat cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KTN1(3895)

General description

Kinectin (KTN1) is a large integral membrane protein present in the endoplasmic reticulum (ER). This protein consists of an N-terminal transmembrane domain and a C-terminal kinesin-binding domain. The KTN1 gene is located on the human chromosome at 14q22.3.

Specificity

Anti-KTN1 (C-terminal) recognizes human KTN1.

Application

Anti-KTN1 (C-terminal) antibody produced in rabbit has been used in:
  • immunocytochemistry
  • immunofluorescence
  • immunoblotting
  • immunoprecipitation

Biochem/physiol Actions

Kinectin (KTN1) protein binds to kinesin and is involved in vesicle transport on microtubules. This protein is also correlated with fibronectin-integrin adhesion sites.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Huan Tran et al.
Journal of cell science, 115(Pt 10), 2031-2040 (2002-04-26)
Integrin receptors mediate the formation of adhesion complexes and play important roles in signal transduction from the extracellular matrix. Integrin-based adhesion complexes (IAC) contain proteins that link integrins to the cytoskeleton and recruit signaling molecules, including vinculin, paxillin, focal adhesion
Derrek P Hibar et al.
Nature, 520(7546), 224-229 (2015-01-22)
The highly complex structure of the human brain is strongly shaped by genetic influences. Subcortical brain regions form circuits with cortical areas to coordinate movement, learning, memory and motivation, and altered circuits can lead to abnormal behaviour and disease. To
Dianne S Schwarz et al.
The Journal of cell biology, 207(1), 41-57 (2014-10-08)
How cells shape and remodel organelles in response to cellular signals is a poorly understood process. Using Xenopus laevis egg extract, we found that increases in cytosolic calcium lead to the activation of an endogenous ribonuclease, XendoU. A fraction of
Lee-Lee Ong et al.
The Journal of biological chemistry, 281(44), 33621-33634 (2006-09-05)
Kinectin is an integral membrane protein with many isoforms primarily found on the endoplasmic reticulum. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta. None of the existing models for the quaternary organization of the elongation
Richik Nilay Mukherjee et al.
Developmental cell, 54(3), 395-409 (2020-05-31)
Nuclear size plays pivotal roles in gene expression, embryo development, and disease. A central hypothesis in organisms ranging from yeast to vertebrates is that nuclear size scales to cell size. This implies that nuclei may reach steady-state sizes set by

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