immunoprecipitation (IP): 5-10 μg using lysate of Jurkat cells indirect immunofluorescence: 2-5 μg/mL using human HeLa cells western blot: 2-4 μg/mL using whole extract of human Jurkat cells
Kinectin (KTN1) is a large integral membrane protein present in the endoplasmic reticulum (ER). This protein consists of an N-terminal transmembrane domain and a C-terminal kinesin-binding domain. The KTN1 gene is located on the human chromosome at 14q22.3.
Specificity
Anti-KTN1 (C-terminal) recognizes human KTN1.
Application
Anti-KTN1 (C-terminal) antibody produced in rabbit has been used in:
Kinectin (KTN1) protein binds to kinesin and is involved in vesicle transport on microtubules. This protein is also correlated with fibronectin-integrin adhesion sites.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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