G6539
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse
clone GFP-20, ascites fluid
Synonym(s):
GFP Antibody - Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse, Gfp Antibody Sigma, Gfp Monoclonal Antibody
Sign Into View Organizational & Contract Pricing
All Photos(6)
About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
GFP-20, monoclonal
mol wt
antigen 27 kDa
contains
15 mM sodium azide
packaging
antibody small pack of 25 μL
technique(s)
dot blot: suitable
indirect ELISA: suitable
western blot: 1:2,000 using purified recombinant GFP preparation
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the GFP-20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a GFP tagged fusion protein. GFP is a 27 kDa (238 amino acid) protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+-activated photoprotein aequorin to GFP. GFP is a unique tool to monitor dynamic processes in a variety of living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. Exogenous substrates and cofactors are not required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP signals can be quantified by flow cytometry, confocal scanning laser microscopy, and fluorometric assays. Indeed, many recombinant proteins have been engineered with GFP tags to facilitate the detection, isolation and purification of the proteins.
The Green Fluorescent Protein (GFP) is derived from the jellyfish Aequorea Victoria and is often used as a gene expression marker. Thus, antibodies to GFP can facilitate the detection and analysis of GFP labeled nucleic acids and proteins. Monoclonal Anti-Green Fluorescent Protein (GFP) antibody is specific for GFP and GFP-labeled biomolecules.
Immunogen
GFP tagged fusion protein
GFP tagged fusion protein.
Application
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody is suitable for use in western blot and ChIP assays.
Monoclonal Anti-Green Fluorescent Protein (GFP) recognizes GFP (27 kDa) using immunoblotting, dot blot, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation and enzyme linked immunosorbent assay (ELISA). The antibody reacts with fusion proteins expressed by prokaryotes expression vectors.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
related product
Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
A myosin IK-Abp1-PakB circuit acts as a switch to regulate phagocytosis efficiency
Dieckmann R, et al.
Molecular Biology of the Cell, 21(9), 1505-1518 (2010)
Justin M O'Sullivan et al.
Nature genetics, 36(9), 1014-1018 (2004-08-18)
Mechanistic analysis of transcriptional initiation and termination by RNA polymerase II (PolII) indicates that some factors are common to both processes. Here we show that two long genes of Saccharomyces cerevisiae, FMP27 and SEN1, exist in a looped conformation, effectively
Gene loops juxtapose promoters and terminators in yeast
O'Sullivan JM, et al.
Nature Genetics, 36(9), 1014-1014 (2004)
Akira Fushiki et al.
eLife, 5 (2016-02-18)
Animals move by adaptively coordinating the sequential activation of muscles. The circuit mechanisms underlying coordinated locomotion are poorly understood. Here, we report on a novel circuit for the propagation of waves of muscle contraction, using the peristaltic locomotion of Drosophila
Yanfeng Dai et al.
The Journal of biological chemistry, 286(22), 19905-19916 (2011-04-05)
CAPRI is a member of the GAP1 family of GTPase-activating proteins (GAPs) for small G proteins. It is known to function as an amplitude sensor for intracellular Ca(2+) levels stimulated by extracellular signals and has a catalytic domain with dual
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service