F3378

Fast Blue BB Salt hemi(zinc chloride) salt

Dye content ≥80 %, Powder

• Synonym(s): 4-Amino-2,5-diethoxybenzanilide diazotated zinc double salt, 4-Benzoylamino-2,5-diethoxybenzenediazonium chloride hemi(zinc chloride) salt
• Linear Formula: C₁₇H₁₈N₃O₃Cl · 1/2 ZnCl₂
• CAS Number: 5486-84-0
• Molecular Weight: 415.94
• Colour Index Number: 37175
• Beilstein/REAXYS Number: 1845288
• EC Number: 226-817-4
• MDL number: MFCD00074765
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894840
• NACRES: NA.47

Properties

• Product Name: Fast Blue BB Salt hemi(zinc chloride) salt, Dye content ≥80 %
• form: powder
• Quality Level: 200
• composition: Dye content, ≥80%
• technique(s): microbe id | staining: suitable
• mp: 157 °C
• solubility: H₂O: 10 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: [Cl-].[Cl-].Cl[Zn]Cl.CCOc1cc([N+]#N)c(OCC)cc1NC(=O)c2ccccc2.CCOc3cc([N+]#N)c(OCC)cc3NC(=O)c4ccccc4
• InChI: 1S/2C17H17N3O3.4ClH.Zn/c2*1-3-22-15-11-14(20-18)16(23-4-2)10-13(15)19-17(21)12-8-6-5-7-9-12;;;;;/h2*5-11H,3-4H2,1-2H3;4*1H;/q;;;;;;+2/p-2
• InChI key: CMFRFQODFZBKTI-UHFFFAOYSA-L

Description

General description

Fast Blue BB Salt hemi(zinc chloride) salt, also known as Cl Azoic diazo 20 (Cl 37175) is a diazonium salt that possesses a lipophilic amine lipophilic with an aromatic system of moderate size. It is commercially available as the zinc chloride double salt.

Application

Fast Blue BB Salt hemi(zinc chloride) salt has been used for alkaline phosphatase staining in cells.[1][2]

Fast blue BB salt is suitable for the staining of enterochromaffin. Its applications are seen in enzyme histochemistry by Gomori and are suitable for the histochemical demonstration of beta-glucuronidase activities, and various peptidases and proteases. It is also used in enzyme histochemistry including the demonstration of serine esterase in blood smears and histological sections. Fast blue BB salt plays a vital role as a routine stain to demonstrate alkaline phosphatase labeled antibodies.

Preparation Note

Purified

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves