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A6930

Sigma-Aldrich

β-N-Acetylhexosaminidase

recombinant, expressed in E. coli, buffered aqueous solution

Synonym(s):

β-N-Acetyl-D-hexosaminide N-acetyl-hexosaminohydrolase

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About This Item

CAS Number:
9012-33-3
Enzyme Commission number:
3.2.1.52 (BRENDA, IUBMB)
EC Number:
232-730-2
MDL number:
MFCD00081402
UNSPSC Code:
12352204

recombinant

expressed in E. coli

form

buffered aqueous solution

UniProt accession no.

P06865

foreign activity

α-Glucosidase <1%
α-mannosidase, β-hexosaminidase, α-fucosidase, neuraminidase, and proteases, none detected (enzyme is expressed in glycosidase-free host)
β-Galactosidase <2%

storage temp.

2-8°C

Gene Information

human ... HEXA(3073)

Biochem/physiol Actions

Active on N-acetyl-β-D-glucosaminides and N-acetyl-β-D-galactosaminides.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at pH 5.0 at 37°C.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl, 10 mM DTT

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
077H1232

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Douglas N Sanders et al.
Molecular genetics and metabolism, 108(1), 70-75 (2012-12-26)
GM2 gangliosidosis is a fatal lysosomal storage disease caused by a deficiency of β-hexosaminidase (EC 3.2.1.52). There are two major isoforms of the enzyme: hexosaminidase A composed of an α and a β subunit (encoded by HEXA and HEXB genes
Federica Vernuccio et al.
Recenti progressi in medicina, 103(12), 559-563 (2012-12-22)
Cardiorenal syndrome is a pathophysiological heart and kidney disorder, in which acute or chronic dysfunction of one organ induces a damage in the other. It's a syndrome more and more often encountered in clinical practice and this implies the need
Tyler Mark Pierson et al.
Molecular genetics and metabolism, 108(1), 65-69 (2012-11-20)
A 12 year-old female presented with a seven-year history of progressive muscle weakness, atrophy, tremor and fasciculations. Cognition was normal. Rectal biopsy revealed intracellular storage material and biochemical testing indicated low hexosaminidase activity consistent with juvenile-onset G(M2)-gangliosidosis. Genetic evaluation revealed
A M Dygai et al.
Bulletin of experimental biology and medicine, 154(2), 278-282 (2013-01-19)
We studied the effect of mobilization of bone marrow multipotent stem cells induced by intragastric administration of pegylated hyaluronate-endo-β-N-acetylhexosaminidase (Peg-HEAHA) on hemopoiesis under conditions of experimental chronic hepatitis. Peg-HEAHA increased the counts of hemopoietic precursors in the hemopoietic tissue against
David J Vocadlo
Current opinion in chemical biology, 16(5-6), 488-497 (2012-11-14)
The addition of N-acetylglucosamine (GlcNAc) O-linked to serine and threonine residues of proteins is known as O-GlcNAc. This post-translational modification is found within multicellular eukaryotes on hundreds of nuclear and cytoplasmic proteins. O-GlcNAc transferase (OGT) installs O-GlcNAc onto target proteins
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