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A2529

Sigma-Aldrich

Alcohol dehydrogenase–Agarose from baker′s yeast (S. cerevisiae)

lyophilized powder

Synonym(s):

ADH, Alcohol:NAD+ oxidoreductase

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About This Item

Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204

form

lyophilized powder

contains

citrate as stabilizer
lactose as stabilizer

storage temp.

−20°C

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Application

Alcohol dehydrogenase may be used to synthesize enantiomerically pure stereoisomers of chiral alcohols. It may be used to study ethanol fuel cells, alcoholism and drug dependence [1]. Product A2529 is from S. Cerevisiae and is an insoluble enzyme with endless applications.

Biochem/physiol Actions

Sigma insoluble enzymes are produced by reacting a soluble enzyme with an inert base. This produces an insoluble compound with the activity of the original enzyme. Alcohol dehydrogenase has broad substrate specificity for alcohols, ketones and acetaldehyde.

Unit Definition

One unit will convert 1.0 μmole of ethanol to acetaldehyde per min at pH 8.8 at 25 °C.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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L S al-Kassim et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 68(6), 907-913 (1990-06-01)
Alcohol dehydrogenase has been purified from the cell-free preparation of Thermoanaerobium brockii to homogeneity, employing combined DEAE, Sephadex, and affinity chromatographic procedures. The enzyme is tetrameric having subunit molecular weight of 40.4 x 10(3). The purified alcohol dehydrogenase is capable
Chien-Ping Chiang et al.
Alcoholism, clinical and experimental research, 36(12), 2047-2058 (2012-12-13)
Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are principal enzymes responsible for metabolism of ethanol (EtOH). Functional polymorphisms of ADH1B, ADH1C, and ALDH2 genes occur among racial populations. This study aimed to systematically determine the functional expressions and cellular localization
Jong Ho Park et al.
Bioscience, biotechnology, and biochemistry, 76(12), 2294-2300 (2012-12-12)
Here, the impact of an extract derived from green tea (Camellia sinensis) and fermentation with Lactobacilli fermentum strain OCS19 was explored with acute alcohol-induced liver damage. The study employed the HepG2 hepatic cell line and an in vivo murine model
N L Vekshin
Biofizika, 57(5), 741-745 (2012-11-10)
The dynamics of proteins, detected by fluorescence, consists of three components: spontaneous dynamics, dipole-dipole photo-induced dynamics, thermal photo-induced dynamics. The photo-induced dynamics can lead to activation as well as inactivation of enzymes.
Yoshiyuki Kimura et al.
Fitoterapia, 84, 163-169 (2012-11-20)
The Curcuma zedoaria rhizome has been used traditionally to treat gastrointestinal diseases as an aromatic stomachic drug, and this is currently used to treat alcohol-induced loss of appetite and nausea in Japan. We examined the effects of various fractions and

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