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T7029

Sigma-Aldrich

Thionin acetate salt

certified by the Biological Stain Commission

Synonym(s):

3,7-Diamino-5-phenothiazinium acetate, Lauth’s violet

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About This Item

Empirical Formula (Hill Notation):
C12H9N3S · C2H4O2
CAS Number:
78338-22-4
Molecular Weight:
287.34
Colour Index Number:
52000
Beilstein/REAXYS Number:
4345073
MDL number:
MFCD00081194
UNSPSC Code:
12171500
PubChem Substance ID:
24900243

grade

certified by the Biological Stain Commission

composition

Dye content, ≥85%

SMILES string

CC([O-])=O.Nc1ccc2nc3ccc(N)cc3[s+]c2c1

InChI

1S/C12H10N3S.C2H4O2/c13-7-1-3-9-11(5-7)16-12-6-8(14)2-4-10(12)15-9;1-2(3)4/h1-6H,13-14H2;1H3,(H,3,4)/q+1;/p-1

InChI key

OWXBIRAFHWASMS-UHFFFAOYSA-M

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Application

For use as a general nuclear stain for chromatin and mucin; for staining Nissl granules by end-point buffering and for showing malignant cells in frozen biopsy sections; and as a stain for semi-thin sections of plant material for light microscopy.

Suitability

Certified for use in Churukian-Schenk thionine method; Stoughton′s method for plant material.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
MKBS9041V
MKBP7371V
MKBL4848V
MKBG6734V
MKBD0109

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Interests in using nanoporous metals for biosensing applications have been increasing. Herein, nanotubular mesoporous PdCu (NM-PdCu) alloy is used to fabricate a novel label-free electrochemical immunosensor for cancer biomarker carcinoembryonic antigen (CEA). It operates through physisorption of anti-CEA on NM-PdCu
Limei Zhu et al.
Biosensors & bioelectronics, 35(1), 507-511 (2012-04-10)
A novel protocol for development of DNA electrochemical biosensor based on thionine-graphene nanocomposite modified gold electrode was presented. The thionine-graphene nanocomposite layer with highly conductive property was characterized by scanning electron microscopy, transmission electron microscopy, cyclic voltammetry and electrochemical impedance
Selvaraj Baskar et al.
Biosensors & bioelectronics, 33(1), 95-99 (2012-01-24)
We report here a novel detection scheme for simultaneous detection of NADH and H(2)O(2) based on a bifunctional poly(thionine)-modified electrode. Electropolymerization of thionine on a "preanodized" screen-printed carbon electrode effectively lowers the oxidation potential of NADH to 0.15 V (vs.
Yunying Xie et al.
Analytica chimica acta, 699(1), 44-48 (2011-06-28)
We reported a graphene-based immunosensor for electrochemical quantification of phosphorylated p53 on serine 15 (phospho-p53(15)), a potential biomarker of gamma-radiation exposure. The principle is based on sandwich immunoassay and the resulting immunocomplex is formed among phospho-p53 capture antibody, phospho-p53(15) antigen
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