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T3405

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

chromogenic, tablet

Synonym(s):

4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

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5 TABS
$61.17
50 TABS
$218.00
100 TABS
$376.00

$61.17

List Price$66.20Save 8%

Available to ship onApril 03, 2025Details

All Photos(1)

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5 TABS
$61.17
50 TABS
$218.00
100 TABS
$376.00

About This Item

Empirical Formula (Hill Notation):
C16H20N2 · 2HCl
CAS Number:
64285-73-0
Molecular Weight:
313.27
EC Number:
264-769-6
MDL number:
MFCD00012961
UNSPSC Code:
12352204
PubChem Substance ID:
24900140
NACRES:
NA.83

$61.17

List Price$66.20Save 8%

Available to ship onApril 03, 2025Details

Product Name

3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet

form

tablet

mp

≥300 °C

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

InChI key

NYNRGZULARUZCC-UHFFFAOYSA-N

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Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
  • anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera[1]
  • anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate[2]
  • in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients[3]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000408058
0000408058
0000379104
0000366382
0000320660

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A novel assay for the evaluation of the prooxidant-antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients
Alamdari DH, et al.
Clinical Biochemistry, 40(3-4), 248-254 (2007)
Darcy S O Mora et al.
Fish & shellfish immunology, 71, 255-263 (2017-09-04)
An experimental contraceptive vaccine was evaluated in Atlantic salmon (Salmo salar). A peptide derived from the beta subunit of luteinizing hormone (LH) was conjugated to two different carrier proteins, bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH), and formulated
C Flohr et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 40(1), 131-142 (2009-09-18)
Observational evidence suggests that infection with helminths protects against allergic disease and allergen skin sensitization. It is postulated that such effects are mediated by helminth-induced cytokine responses, in particular IL-10. We tested this hypothesis in a rural area of central
Trisha T N Tay et al.
Zoo biology, 37(2), 90-97 (2018-04-18)
The female giant panda's estrus is known to be photoperiod sensitive, triggered by increasing day length. A pair of giant pandas was brought to Singapore in September 2012 and exposed to a constant temperature and photoperiod during the first 2
Olga D Hendrickson et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1141, 122014-122014 (2020-02-23)
The aim of this study was to develop a rapid and sensitive immunochromatographic test system for the detection of lincomycin (LIN), which belongs to the lincosamide group of antibiotics and contaminates food products of animal origin. Two formats of immunochromatographic
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