Safranin O stain for cartilage requires a specific procedure involving fixative, deparaffinization, staining, and mounting. The use of acetic acid after staining sets the stain to the tissue.
S8884
Safranin O
Dye content, ≥80%, certified by the Biological Stain Commission, powder
Synonym(s):
Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
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25 G
$124.00
100 G
$432.00
About This Item
Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
Molecular Weight:
350.84
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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Product Name
Safranin O, certified by the BSC, certified by the Biological Stain Commission
grade
certified by the Biological Stain Commission
agency
certified by the BSC
form
powder
composition
Dye content, ≥80%
technique(s)
microbe id | staining: suitable
color
green to very dark green
solubility
H2O: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
InChI
1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
InChI key
OARRHUQTFTUEOS-UHFFFAOYSA-N
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General description
Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.[1][2]
Application
Suitability
Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Is there a staining protocol available for staining cartilage, particularly for decalcified bone (joint tissue) using CalX, and subsequent staining with Fast green and Safranin O?
1 answer-
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