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Key Documents

S7903

Sigma-Aldrich

Sucrose

≥99.5% (GC), BioXtra

Synonym(s):

α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar

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About This Item

Empirical Formula (Hill Notation):
C12H22O11
CAS Number:
Molecular Weight:
342.30
Beilstein/REAXYS Number:
90825
EC Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.21

biological source

sugar cane

Quality Level

product line

BioXtra

assay

≥99.5% (GC)

form

crystals

optical activity

[α]20/D +66.5±1°, c = 10 in H2O(lit.)

technique(s)

gas chromatography (GC): suitable

impurities

Insoluble matter, passes filter test

ign. residue

≤0.01% (as SO4)

loss

≤0.2% loss on drying, HV, 18 - 26°C

color

white

pH

5.5-7.5 (20 °C, 1 M in H2O)

mp

185-187 °C (lit.)

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.005%
sulfate (SO42-): ≤0.005%

cation traces

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorption

≤0.07 at 280 in H2O at 1 M
≤0.09 at 260 in H2O at 1 M

SMILES string

OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1

InChI key

CZMRCDWAGMRECN-UGDNZRGBSA-N

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Application

Sucrose may be used to prepare density gradients for cell/organelle separation. The refractive index of the solution will be directly related to the density. In addition, sucrose can be used as a supplement in plant, insect, and bacterial culture media, and in various enzymatic assays.

Preparation Note

Solutions can be autoclaved for 15-20 minutes at a maximum of 121 °C. There will be some hydrolysis to glucose and fructose, depending in part on how rapidly the autoclave comes to the required temperature and pressure. Care must be taken to prevent solutions from caramelizing.

Other Notes

To gain a comprehensive understanding of our extensive range of Disaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Rickwood, D., ed.
Centrifugation - a practical approach, Appendix IV-Appendix IV (1984)
Dawson, R.M.C., et al.
Data for Biochemical Research, 545-546 (1986)
Lara Hassan et al.
mBio, 10(4) (2019-07-04)
It is essential for microbes to acquire information about their environment. Fungi use soluble degradation products of plant cell wall components to understand the substrate composition they grow on. Individual perception pathways have been well described. However, the interconnections between
Julia B Cordero et al.
The EMBO journal, 33(13), 1474-1491 (2014-05-03)
The non-receptor tyrosine kinase c-Src, hereafter referred to as Src, is overexpressed or activated in multiple human malignancies. There has been much speculation about the functional role of Src in colorectal cancer (CRC), with Src amplification and potential activating mutations
Denise Harrison et al.
Pediatrics, 130(5), 918-925 (2012-10-10)
The use of oral sucrose has been the most extensively studied pain intervention in newborn care to date. More than 150 published studies relating to sweet-taste-induced calming and analgesia in human infants have been identified, of which 100 (65%) include

Protocols

Enzymatic Assay of Invertase

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