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RAB0540

Sigma-Aldrich

Human FGF19 / Fibroblast Growth Factor 19 ELISA Kit

for serum, plasma, cell culture supernatants and urine

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type urine
sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 30 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FGF19(9965)

General description

This ELISA antibody pair detects human Fibroblast Growth Factor 19.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Fibroblast growth factor (FGF) 19 controls bile acid, fatty acid, glucose and phosphate metabolism in target organs by activating FGF receptor 4. FGF19 regulates hepatic protein and glycogen metabolism via activation of insulin-independent endocrine pathway. In diabetic mice, FGF19 reduces the levels of serum glucose and triglycerides. Overexpression of the gene leads to the development of hepatocellular carcinoma (HCC).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable


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H Johansson et al.
Journal of internal medicine, 287(5), 534-545 (2020-01-25)
Fibroblast growth factor 19 (FGF19) is produced in the small intestine and is involved in suppression of hepatic bile acid (BA) synthesis. FGF19 is also expressed in the liver and serum levels are elevated in adults with cholestatic liver disease.
Kai Wang et al.
Cell reports, 26(1), 222-235 (2019-01-04)
We demonstrated the metabolic benefits of Parabacteroides distasonis (PD) on decreasing weight gain, hyperglycemia, and hepatic steatosis in ob/ob and high-fat diet (HFD)-fed mice. Treatment with live P. distasonis (LPD) dramatically altered the bile acid profile with elevated lithocholic acid (LCA)
José O Alemán et al.
Journal of translational medicine, 16(1), 244-244 (2018-09-05)
Microbiota and bile acids in the gastrointestinal tract profoundly alter systemic metabolic processes. In obese subjects, gradual weight loss ameliorates adipose tissue inflammation and related systemic changes. We assessed how rapid weight loss due to a very low calorie diet
Samer Al-Dury et al.
Journal of hepatology, 71(5), 986-991 (2019-06-30)
The nuclear farnesoid X receptor (FXR) agonist obeticholic acid (OCA) has been developed for the treatment of liver diseases. We aimed to determine whether OCA treatment increases the risk of gallstone formation. Twenty patients awaiting laparoscopic cholecystectomy were randomized to
Meilin Wei et al.
EBioMedicine, 55, 102766-102766 (2020-05-15)
The composition of the bile acid (BA) pool is closely associated with obesity and is modified by gut microbiota. Perturbations of gut microbiota shape the BA composition, which, in turn, may alter important BA signaling and affect host metabolism. We

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