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RAB0071

Sigma-Aldrich

Mouse CTACK / CCL27 ELISA Kit

for serum, plasma and cell culture supernatant

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 24.69-18000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Ccl27(20301)

General description

The Mouse CTACK ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CTACK in serum, plasma, cell culture supernatants and urine.
The CCL27 (C-C motif chemokine ligand 27) gene codes for a T cell skin-homing chemokine and is expressed in epidermal keratinocytes. It is also referred to as cutaneous T cell-attracting chemokine (CTACK).

Immunogen

Recombinant Mouse CTACK

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

CCL27 (C-C motif chemokine ligand 27) contributes to the progression of inflammatory skin diseases. In animal models, atopic dermatitis is known to increase the CCL27 gene expression. The CCL27 gene is downregulated in cancer. It regulates the enrollment of T cells to inflamed skin.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials


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CCL27?transgenic mice show enhanced contact hypers ensitivity to Th2, but not Th1 stimuli.
Kagami S, et al.
European Journal of Immunology, 38(3), 647-657 (2008)
CCL27 is a critical factor for the development of atopic dermatitis in the keratin-14 IL-4 transgenic mouse model.
Chen L, et al.
International Immunology, 18(8), 1233-1242 (2006)
Tumor immune escape by the loss of homeostatic chemokine expression.
Pivarcsi A, et al.
Proceedings of the National Academy of Sciences of the USA, 104(48), 19055-19060 (2007)

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