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P7643

Sigma-Aldrich

PIPES dipotassium salt

≥99% (titration)

Synonym(s):

1,4-Piperazinediethanesulfonic acid dipotassium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) dipotassium salt

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About This Item

Empirical Formula (Hill Notation):
C8H16K2N2O6S2
CAS Number:
108321-27-3
Molecular Weight:
378.55
MDL number:
MFCD00069751
UNSPSC Code:
12161700
PubChem Substance ID:
24898738
NACRES:
NA.25

Quality Level

200

assay

≥99% (titration)

form

crystalline powder

useful pH range

6.1-7.5

pKa (25 °C)

6.8

solubility

water: 0.25 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

[K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;;/h1-8H2,(H,11,12,13)(H,14,15,16);;/q;2*+1/p-2

InChI key

BJQYFCAMUXGZFN-UHFFFAOYSA-L

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General description

PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.

Application

PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Use of single molecule fluorescence polarization microscopy to study protein conformation and dynamics of kinesin-microtubule complexes
Benoit MPMH and Sosa H
Single Molecule Analysis, 15(1), 199-216 (2018)
Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation
Nie J, et al.
BMC Developmental Biology, 15(1), 10-10 (2015)
Dynamic phosphorylation of NudC by Aurora B in cytokinesis
Weiderhold KN
PLoS ONE, 11(4), e0153455-e0153455 (2016)
Helical alignment inversion of microtubules in accordance with a structural change in their lattice
Shikinaka K, et al.
Soft Matter, 11(19), 3869-3874 (2015)
Nina Marie Pedersen et al.
The Journal of cell biology, 219(8) (2020-06-02)
Cancer cells break tissue barriers by use of small actin-rich membrane protrusions called invadopodia. Complete invadopodia maturation depends on protrusion outgrowth and the targeted delivery of the matrix metalloproteinase MT1-MMP via endosomal transport by mechanisms that are not known. Here

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