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P6186

Sigma-Aldrich

Thidiazuron

BioReagent, suitable for plant cell culture

Synonym(s):

1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea, TDZ

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About This Item

Empirical Formula (Hill Notation):
C9H8N4OS
CAS Number:
Molecular Weight:
220.25
Beilstein/REAXYS Number:
1078092
EC Number:
MDL number:
UNSPSC Code:
10171502
PubChem Substance ID:
NACRES:
NA.72

product line

BioReagent

Quality Level

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

SMILES string

O=C(Nc1ccccc1)Nc2cnns2

InChI

1S/C9H8N4OS/c14-9(12-8-6-10-13-15-8)11-7-4-2-1-3-5-7/h1-6H,(H2,11,12,14)

InChI key

HFCYZXMHUIHAQI-UHFFFAOYSA-N

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Application

Thidiazuron has been used:
  • as a component of the proliferation medium to test its effect on the proliferation of embryo-like structures obtained from the post-culture of shoot tips
  • as a growth regulator in Murashige and Skoog (MS) media to culture the nodal segments of Hyssopus officinalis L. seedlings
  • as a cytokinin in induction media to experiment with the hormonal composition

Biochem/physiol Actions

Thidiazuron (TDZ) is a plant growth regulator and a synthetic diphenylurea cytokinin used in micropropagations to supplement media such as Murashige and Skoog medium. TDZ promotes plant organogenesis (shoot regeneration) and plant regeneration. Thidiazuron (TDZ) enables the production of virus-free stock plants, speeds up breeding, and provides new genotypes for the market. It is also used as a phenylurea herbicide for controlling weeds in agricultural and nonagricultural practices. Thidiazuron acts as a pesticide by inhibiting photosystem II.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Exclamation markEnvironment

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certificates of Analysis (COA)

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María Laura Fontana et al.
Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al, 33(3), 179-186 (2010-01-14)
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: alpha-naphthalenacetic
Wanwimon Lualon et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 63(1-2), 96-100 (2008-04-05)
An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem explants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The
Dmytro P Yevtushenko et al.
Plant cell reports, 29(3), 211-221 (2010-01-21)
Many economically important species of Populus, especially those in sections Aigeiros and Tacamahaca, remain recalcitrant to genetic transformation. In this study, a simple and reliable protocol was developed for the efficient Agrobacterium-mediated transformation of a difficult-to-transform, but commercially viable, hybrid
Lucas Cutri et al.
Mechanisms of development, 130(1), 61-69 (2012-06-05)
Tendrils can be found in different plant species. In legumes such as pea, tendrils are modified leaves produced by the vegetative meristem but in the grape vine, a same meristem is used to either form a tendril or an inflorescence.
Hemaid Ibrahim Soliman et al.
GM crops, 1(1), 40-51 (2010-01-01)
Fig is one of the most important fruit trees in Egypt. It used to constitute the major source of income for the inhabitants of the western north coast of Egypt. Since 1993 fig cultivations were threatened by a number of

Protocols

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

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