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N3254

Sigma-Aldrich

4-Nitrophenyl phosphate di(tris) salt

phosphatase substrate, ≥97% (enzymatic), powder

Synonym(s):

4-Nitrophenyl phosphate bis(tris) salt

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1 G
$52.38
5 G
$89.37
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$357.30
100 G
$918.00

$52.38

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1 G
$52.38
5 G
$89.37
25 G
$357.30
100 G
$918.00

About This Item

Linear Formula:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS Number:
68189-42-4
Molecular Weight:
461.36
Beilstein/REAXYS Number:
1578142
EC Number:
269-198-6
MDL number:
MFCD00037009
UNSPSC Code:
12352204
PubChem Substance ID:
24897633
NACRES:
NA.83

$52.38

List price$58.20Save 10%
Web-Only Promotion

Available to ship onApril 08, 2025Details

Product Name

4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

assay

≥97% (enzymatic)

form

powder

solubility

water: 50 mg/mL, clear, colorless to light yellow

Storage temp.

−20°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

Inchi Key

XXAXKCWOTRABOW-UHFFFAOYSA-N

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Application

4-Nitrophenyl phosphate di(tris) salt has been used:
  • as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates[1]
  • to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)[2]
  • for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)[2]

Biochem/physiol Actions

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.[3]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN1064
BCCJ4964
BCCG8128
BCBX4816
BCBS1398V

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Mary E Wood et al.
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B Le Gars Santoni et al.
Bioactive materials, 10, 222-235 (2021-12-14)
β-Tricalcium Phosphate (β-TCP), one of the most used bone graft substitutes, may contain up to 5 wt% foreign phase according to standards. Typical foreign phases include β-calcium pyrophosphate (β-CPP) and hydroxyapatite (HA). Currently, the effect of small amounts of impurities on
B Le Gars Santoni et al.
Bioactive materials, 10, 222-235 (2021-12-14)
β-Tricalcium Phosphate (β-TCP), one of the most used bone graft substitutes, may contain up to 5 wt% foreign phase according to standards. Typical foreign phases include β-calcium pyrophosphate (β-CPP) and hydroxyapatite (HA). Currently, the effect of small amounts of impurities on
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