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MAK044

Sigma-Aldrich

Free Fatty Acid Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Free Fatty Acid Assay Kit

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About This Item

UNSPSC Code:
12161503

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

General description

Free fatty acids, also know as nonesterified fatty acids, circulate in the plasma bound to albumin. The majority of free fatty acids are derived from either dietary sources or are mobilized from adipose tissue. Plasma free fatty acids are elevated in many obesity-related disorders and may contribute to insulin resistance in peripheral tissues. Conditions such as sepsis and tumors producing lipoactive homones may also be associated with elevated free fatty acid levels.

Abnormalities in fatty acid metabolism causes fatty liver disease.[1] Accumulation of free fatty acid in myocytes leads to the risk of cardiac dysfunction.[2] Increased fatty acid β-oxidation is implicated in cardiac ischemia.[2]

Application

Free Fatty Acid Quantitation Kit has been used in the following:
  • to measure lipid hydrolysis, by determining the free fatty acid in digested sample.[3]
  • to measure plasma and serum free fatty acids level.[4][5]
  • to quantify free fatty acids level in liver tissue.[6]

Suitability

Suitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc.

Principle

In this kit, the concentration of fatty acids (C8 and longer) is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the fatty acids present.

Related product

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8L11K06120
8A17K06120
8C21K06120
8E18K06120
8B15K06120

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Association of Temperament and acute stress responsiveness with Productivity, Feed efficiency, and Methane emissions in Beef cattle: an Observational study
Llonch P, et al.
Frontiers in veterinary science, 3, 43-43 (2016)
Transgenic expression of fatty acid transport protein 1 in the heart causes lipotoxic cardiomyopathy.
Chiu H C, et al.
Circulation Research, 96(2), 225-233 (2005)
Sarmistha Mukherjee et al.
Hepatology (Baltimore, Md.), 65(2), 616-630 (2016-11-04)
The regenerative capacity of the liver is essential for recovery from surgical resection or injuries induced by trauma or toxins. During liver regeneration, the concentration of nicotinamide adenine dinucleotide (NAD) falls, at least in part due to metabolic competition for
Estrogen Receptor Involvement in Noradrenergic Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neurotransmitter and Stimulus-Specific Glycogen Phosphorylase Enzyme Isoform Expression.
A S M H Mahmood et al.
ASN neuro, 12, 1759091420910933-1759091420910933 (2020-04-03)
Changes in feed intake during isolation stress in respiration chambers may impact methane emissions assessment.
Llonch P, et al.
Animal Production Science, 58(6), 1011-1016 (2018)
View All Related Papers

Questions

1–9 of 9 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Is there a step in the process where samples can be preserved for future analysis using the kit?

    1 answer

    1. Yes, samples can be preserved for future analysis using the kit by aliquoting and freezing them if needed for multiple uses. However, it's recommended to use fresh samples whenever possible to ensure accurate results. Dried lipids can be stored in 200 µl of Fatty Acid Assay Buffer at -80°C. In case of lipid precipitation, re-dissolve the lipids by sonication. However, the supplier does not specify the maximum duration for which the samples stored at -80°C will remain stable. Storing samples at -80°C may impact their stability, potentially resulting in lower than expected readings. Therefore, it is recommended to use fresh samples for this assay whenever possible to ensure accurate results.
      If frozen samples are used, they should be snap frozen in liquid nitrogen immediately after collection. These samples cannot undergo multiple freeze-thaw cycles and must be stored for relatively short periods. It's important to note that stability studies on different sample types have not been conducted, so there is no advice available regarding the timeframe for measuring frozen samples.

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  4. Can MAK044 quantify only albumin-bound free fatty acids (FFA), or does it also quantify unbound FFA?

    1 answer

    1. The kit specifically detects unbound free fatty acids in the samples and does not detect any albumin-bound free fatty acids.

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  5. What are the recommended storage instructions for the Enhancer reagent and unused Assay Buffer in the MAK044 Free Fatty Acid Quantitation Kit?

    1 answer

    1. The enhancer can be aliquoted and stored at -20°C, while the assay buffer can also be stored in aliquots at -20°C to avoid multiple freeze-thaw cycles.

      Helpful?

  6. I would like to quantify butyric acid in bacteria culture supernatant. Does this MAK044 Free Fatty Acid Quantitation Kit suitable or is there a kit you can recommend. Thank you. 

    1 answer

    1. Butyric acid is a short-chain fatty acid. This kit is suitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma, and other body fluids, food, growth media, etc. The Short Chain Fatty Acid Kit, prod number SBR0030, offers a potential analytical option. This kit is suitable for detection of several SCFAs, including Butyric, by HPLC, LC-MS, and GC-MS. See the link below to the technical bulleting should these methods be suitable:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

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  7. Can this kit be used to analyse NEFA in plasma using the Pentra C400 instrument?

    1 answer

    1. This kit is suitable for detecting nonesterified fatty acids in plasma (NEFA). However, suitability with Pentra C400 instrument has not been determined. This application must be determined by the end-user.

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  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  9. Can these kits be used with serum samples from mouse?

    1 answer

    1. Yes, this kit can be used on mouse serum samples. We generally recommend that fresh serum samples be used. Frozen samples can probably be used. However, the successful use of frozen samples depends on the efficiency of freezing them and the amount of time they have been frozen. If previously frozen serum samples are used, you may want to check the performance of the kit with fresh vs. frozen samples.

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