Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

FBS25

Sigma-Aldrich

Fast Blue RR Salt

Preweighed Capsules

Synonym(s):

4-Benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt, Azoic Diazo No. 24

Sign Into View Organizational & Contract Pricing


About This Item

Linear Formula:
C15H14ClN3O3 · 1/2 ZnCl2
CAS Number:
Molecular Weight:
387.89
Colour Index Number:
37155
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

Quality Level

form

powder

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1NC(=O)c2ccccc2.COc3cc([N+]#N)c(OC)cc3NC(=O)c4ccccc4

InChI

1S/2C15H13N3O3.4ClH.Zn/c2*1-20-13-9-12(18-16)14(21-2)8-11(13)17-15(19)10-6-4-3-5-7-10;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2

InChI key

LLARVKAOQLUCQI-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

General description

Fast Blue RR is part of the azo coupling system and is required for the staining of hydrolytic enzymes. In the presence of naphthol, it results in the formation of an insoluble coloured precipitate at the site of enzyme activity.

Application

Used as a kit component for the determination of alkaline phosphatase in Sigma Kits 85L1 and 85L2 and for the determination of nonspecific esterase in Sigma Kit 90A1.
Fast Blue RR Salt has been used for the staining of alkaline phosphatase activity in cells.

Packaging

Actual weight of the capsule will vary with dye lot and has been optimized by assay.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Manchenko GP
Handbook of Detection of Enzymes on Electrophoretic Gels (2002)
Emergence of patterned stem cell differentiation within multicellular structures.
Ruiz SA and Chen CS
Stem Cells, 26, 2921-2921 (2008)
Jung-Min Kim et al.
Nature communications, 11(1), 2289-2289 (2020-05-10)
The osteoblast differentiation capacity of skeletal stem cells (SSCs) must be tightly regulated, as inadequate bone formation results in low bone mass and skeletal fragility, and over-exuberant osteogenesis results in heterotopic ossification (HO) of soft tissues. RUNX2 is essential for
Argiris Papathanasopoulos et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 29(9), 1327-1335 (2011-03-25)
An unexpected side effect of some classes of anticoagulants has been osteoporosis which may be, at least in part, related to deranged mesenchymal stem cell (MSC) function. The aim of the present study was to compare the effect of fondaparinux

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service