93426

Triton^™ X-100

BioXtra, for molecular biology

• Synonym(s): t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
• Linear Formula: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• CAS Number: 9036-19-5
• Beilstein/REAXYS Number: 2315025
• MDL number: MFCD00128254
• UNSPSC Code: 12352200
• PubChem Substance ID: 329770232

Properties

• grade: for molecular biology
• description: non-ionic
• product line: BioXtra
• form: liquid
• technique(s): flow cytometry: suitable; immunofluorescence: suitable; permeability assay: suitable
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• ign. residue: ≤0.2%
• transition temp: cloud point 65 °C; pour point ~7 °C
• solubility: H₂O: 0.005 M at 20 °C, clear, colorless
• density: 1.065 g/mL at 20 °C
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤500 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Na: ≤500 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤5 mg/kg
• storage temp.: 2-8°C
• SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• InChI key: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Triton^™ X-100 is a non-ionic surfactant that aids the recovery of membrane-bound proteins under mild non-denaturing conditions.

Application

Suitable for extraction of membrane-bound proteins

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Principle

Triton^™ X-100 compromises the integrity of the lipid bilayer by disrupting the polar head group on the hydrogen bonding. The detergent monomer inserts itself into the lipid bilayer resulting in over-permeabilization and disruption of the cell structure.[1]

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• pictograms: GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H315,H318,H410
• pcodes: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: 483.8 °F - closed cup
• flash_point_c: 251 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter