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93356

Sigma-Aldrich

Tricine

BioUltra, ≥99.5% (NT)

Synonym(s):

N-[Tris(hydroxymethyl)methyl]glycine

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About This Item

Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
5704-04-1
Molecular Weight:
179.17
Beilstein/REAXYS Number:
1937804
EC Number:
227-193-6
MDL number:
MFCD00004277
UNSPSC Code:
12161700
PubChem Substance ID:
24889851

product line

BioUltra

assay

≥99.5% (NT)

impurities

Insoluble matter, passes filter test

ign. residue

≤0.05% (as SO4)

loss

≤0.5% loss on drying, 110 °C

pH

4.5-6.0 (25 °C, 0.5 M in H2O)

useful pH range

7.4-8.8

pKa (25 °C)

8.1

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤500 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.03
λ: 280 nm Amax: 0.02

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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Application

Buffer component for separation of low molecular weight peptides.

Biochem/physiol Actions

Tricine, a buffer with a useful range from 7.4 to 8.8, is commonly used for the electrophoretic separation of low molecular weight proteins in gels and membranes. It is an effective buffer for ATP assays using firefly luciferase. Tricine is also an effective hydroxyl radical scavenger used to protect molecules during radical generating procedures such as irradiation.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCBJ8261V
BCBB6474V
BCBD3417V
BCBB6474
1452534

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J.J. Webster et al.
Journal of Applied Biochemistry, 2, 469-469 (1980)
J Ambler et al.
Clinical chemistry, 26(8), 1221-1223 (1980-07-01)
Two new non-barbiturate buffers have been formulated for serum protein electrophoresis on cellulose acetate membranes. Both buffers give five distrinct fractions and are suitable for all systems, but have been primarily designed to meet the needs of the Gelman "Sepratek"
Thierry Rabilloud
Journal of proteomics, 73(8), 1562-1572 (2010-04-17)
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
Christian Nilsson et al.
Electrophoresis, 31(3), 459-464 (2010-02-02)
Totally porous lipid-based liquid crystalline nanoparticles were used as pseudostationary phase for capillary electroseparation with LIF detection of proteins at physiological conditions using unmodified cyclic olefin copolymer capillaries (Topas, 6.7 cm effective length). In the absence of nanoparticles, i.e. in
Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
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