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90909C

Sigma-Aldrich

HEPES

dry powder

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
7365-45-9
Molecular Weight:
238.30
Beilstein/REAXYS Number:
883043
EC Number:
230-907-9
MDL number:
MFCD00006158
UNSPSC Code:
12352200
PubChem Substance ID:
329769965

description

for research or for further manufacturing use

form

dry powder

useful pH range

6.8-8.2

pKa (25 °C)

7.5

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Application

HEPES is added in many cell culture media. It was used to prepare cell suspension for flow cytometry analysis of cardiac progenitor cells.[1]
HEPES is used in many media because it has more buffering capacity in the physiologic pH range of 7.2 - 7.4 at 37°C than sodium bicarbonate. It is commonly added at concentrations between 10 mM and 25 mM. Higher levels cause cytotoxicity. Because bicarbonate is nutritionally necessary for most cells, HEPES should be added in addition to, not in place of, sodium bicarbonate.

Biochem/physiol Actions

HEPES has buffering capacity in the pH range of 7.2 - 7.4. It is added frequently to cell culture media in addition to sodium bicarbonate at 10 mM to 25 mM concentration.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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