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78449

o-Phenylenediamine dihydrochloride

Name: <I>o</I>-Phenylenediamine dihydrochloride
Brand: SIGMA

tablet, ~30 mg substrate per tablet

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About This Item

Linear Formula:

C₆H₄(NH₂)₂·2HCl

CAS Number:

615-28-1

Molecular Weight:

181.06

Beilstein/REAXYS Number:

3912045

EC Number:

210-418-7

MDL number:

MFCD00012966

UNSPSC Code:

12352200

PubChem Substance ID:

24887307

This product is discontinued. Contact Technical Servicefor assistance.

form

tablet

mp

258 °C (dec.) (lit.)

solubility

H₂O: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

Phenylenediamine (O-Phenylenediamine) may be used in a wide variety of peroxidase based chromogenic assays and detection systems such as those based upon peroxidase conjugated primary and secondary antibodies.

pictograms

GHS06,GHS08,GHS09

signalword

Danger

hcodes

H301,H312 + H332,H317,H319,H341,H351,H410

pcodes

P273 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

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pH-tuned metal coordination and peroxidase activity of a peptide dendrimer enzyme model with a Fe(ii)bipyridine at its core.

Geotti-Bianchini P, Darbre T, Reymond JL.

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Matthew A Wozniak et al.

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A role for nucleotides in support of breast cancer angiogenesis: heterologous receptor signalling.

N Yokdang et al.

British journal of cancer, 104(10), 1628-1640 (2011-04-21)

Human breast carcinoma cells secrete an adenosine 5'-diphosphate transphosphorylase (sNDPK) known to induce endothelial cell tubulogenesis in a P2Y receptor-dependent manner. We examined sNDPK secretion and its effects on human endothelial cells. Nucleoside diphosphate kinase (NDPK) secretion was measured by

Wide cross-reactivity between Anopheles gambiae and Anopheles funestus SG6 salivary proteins supports exploitation of gSG6 as a marker of human exposure to major malaria vectors in tropical Africa.

Cinzia Rizzo et al.

Malaria journal, 10, 206-206 (2011-07-29)

The Anopheles gambiae gSG6 is an anopheline-specific salivary protein which helps female mosquitoes to efficiently feed on blood. Besides its role in haematophagy, gSG6 is immunogenic and elicits in exposed individuals an IgG response, which may be used as indicator

Spectrophotometric quantification of lactose in solution with a peroxidase-based enzymatic cascade reaction system.

Sara Fornera et al.

Analytical and bioanalytical chemistry, 401(7), 2307-2310 (2011-08-17)

A spectrophotometric assay was developed for the quantification of lactose in aqueous solution via a one-pot enzymatic cascade reaction at 25 °C and pH 7.2. Lactose (0.2-1.8 mM), E. coli β-galactosidase (β-Gal), Aspergillus niger glucose oxidase (GOD), horseradish peroxidase (HRP)

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