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74092

Sigma-Aldrich

Sodium phosphate monobasic solution

BioUltra, 5 M in H2O

Synonym(s):

Monosodium phosphate solution, Sodium dihydrogen phosphate, Sodium dihydrogen orthophosphate,Sodium orthophosphate monobasic, Dihydrogen monosodium phosphate, Dihydrogen sodium phosphate

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About This Item

Empirical Formula (Hill Notation):
H2NaO4P
CAS Number:
Molecular Weight:
119.98
Beilstein/REAXYS Number:
3903772
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

product line

BioUltra

form

liquid

concentration

5 M in H2O

impurities

insoluble matter, passes filter test
≤0.001% total nitrogen (N)

pH

3.0-4.5 (25 °C, 5 M in H2O)

solubility

H2O: 5 M, clear, colorless

density

1.40 g/mL at 20 °C

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤30 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.025
λ: 280 nm Amax: ≤0.02

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].OP(O)([O-])=O

InChI

1S/Na.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

AJPJDKMHJJGVTQ-UHFFFAOYSA-M

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General description

Sodium phosphate monobasic serves as a source of phosphate ions. The use of sodium phosphate monobasic to correct severe hypophosphatemia conditions has been reviewed.

Application

Sodium phosphate monobasic solution has been used as a component of aCSF (artificial cerebrospinal fluid) stock solution for use in the patch-seq analysis of small interneurons. It has also been used as a supplement in the preparation of crude extract of cells for decentralizing cell-free RNA sensing.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Decentralizing Cell-Free RNA Sensing With the Use of Low-Cost Cell Extracts
Arce A, et al.
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Marcela Lipovsek et al.
STAR protocols, 1(3), 100146-100146 (2020-12-31)
Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized demands
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The aim of this study was the development of poly(D,L-lactide-co-glycolide) (PLGA) microspheres with controlled porosity, to obtain microspheres that afford continuous release of a macromolecular model compound (blue dextran). PLGA microspheres with a size of around 40 μm and narrow size

Protocols

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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