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56900

Sigma-Aldrich

Fluorescent Red Mega 500

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Empirical Formula (Hill Notation):
C31H36N2O8S
CAS Number:
Molecular Weight:
596.69
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:

product line

BioReagent

assay

≥90% (HPLC)

form

solid

fluorescence

λex 508 nm; λem 608 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CCN(CC)c1ccc2C(O)=C(\C=C\C3=[N+](CCCCCC(O)=O)c4ccc(cc4C3(C)C)S([O-])(=O)=O)C(=O)Oc2c1

InChI

1S/C31H36N2O8S/c1-5-32(6-2)20-11-13-22-26(18-20)41-30(37)23(29(22)36)14-16-27-31(3,4)24-19-21(42(38,39)40)12-15-25(24)33(27)17-9-7-8-10-28(34)35/h11-16,18-19H,5-10,17H2,1-4H3,(H2,34,35,38,39,40)

InChI key

ACILOXWOYCJZEB-UHFFFAOYSA-N

General description

Mega dyes are specifically designed for multicolor techniques. Fluorescent red Mega 500 is characterized by an extremely large stoke′s shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength light sources and can be excited using the same light source or filter settings as for fluorescein and other widely used dyes.

Application

Fluorescent red Mega 500 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stoke′s shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This means that it can be excited with the same light source or filter arrangement than fluorescein and other widely used dyes as well as other Mega dye. Due to the different emission wavelength several different labels can be detected independently in the same experiment. Due to the small size of the Mega-labels and the direct conversion of excitation light into emission without using a FRET mechanism we foresee a variety of possible applications including DNA sequencing and FISH microscopy.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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