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49328

Sigma-Aldrich

EVOblue 30

for fluorescence, ≥90.0% (coupling to amines)

Synonym(s):

3-[N-(2-Carboxyethyl)methylamino]-7-[N-ethyl(3-sulfonatopropyl)amino]phenoxazin-5-ium

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About This Item

Empirical Formula (Hill Notation):
C21H25N3O6S
CAS Number:
Molecular Weight:
447.50
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:

grade

for fluorescence

assay

≥90.0% (coupling to amines)

solubility

DMF: soluble

fluorescence

λex 635 nm; λem 666 nm in 0.1 M phosphate pH 7.0

storage temp.

−20°C

SMILES string

CCN(CCCS([O-])(=O)=O)c1ccc2nc3ccc(cc3[o+]c2c1)N(C)CCC(O)=O

InChI

1S/C21H25N3O6S/c1-3-24(10-4-12-31(27,28)29)16-6-8-18-20(14-16)30-19-13-15(5-7-17(19)22-18)23(2)11-9-21(25)26/h5-8,13-14H,3-4,9-12H2,1-2H3,(H-,25,26,27,28,29)

InChI key

RCTKCVWZEGPPCK-UHFFFAOYSA-N

Application

EVOblue 30 is a fluorescent dye for covalently labeling a wide range of biological molecules. The lower excitation energy needed for EVOblue 30 causes less photodestruction and exceeds the lifetime of the fluorescent signal compared to common dyes. EVOblue 30 is optimized for an excitation at 635 nm and an emission around 670 nm - both allowing the use of common laser and filter sets. In contrast to commercially available red excitable dyes, EVOblue 30 reveals a high chemical stability and suitability for high performance fluorescence applications.

Features and Benefits

EVOblue® 30 is a fluorescent dye for covalently labeling a wide range of biological molecules. The lower excitation energy needed for EVOblue 30 causes less photodestruction and exceeds the lifetime of the fluorescent signal compared to common dyes. EVOblue 30 is optimized for an excitation at 635 nm and an emission around 670 nm - both allowing the use of common laser and filter sets. In contrast to commercially available red excitable dyes, EVOblue 30 reveals a high chemical stability and suitablity for high performance fluorescence applications.

Other Notes

Fluorescent marker for the red spectral region which is very stable[1]

Legal Information

EVOblue is a trademark of Evotec Technologies GmbH

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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C.E. Lopez, J. Fries
Bioforum, 24, 32-32 (2001)

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