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Eosin diacetate

Name: Eosin diacetate
Brand: SIGMA

for fluorescence, ≥98.0% (TLC)

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About This Item

Empirical Formula (Hill Notation):

C₂₄H₁₂Br₄O₇

CAS Number:

7284-92-6

Molecular Weight:

731.96

Beilstein/REAXYS Number:

71271

MDL number:

MFCD00037519

UNSPSC Code:

12352200

PubChem Substance ID:

57650267

grade

for fluorescence

assay

≥98.0% (TLC)

solubility

DMF: soluble
acetone: soluble

suitability

in accordance for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)Oc1c(Br)cc2c(Oc3c(Br)c(OC(C)=O)c(Br)cc3C24OC(=O)c5ccccc45)c1Br

InChI

1S/C24H12Br4O7/c1-9(29)32-21-15(25)7-13-19(17(21)27)34-20-14(8-16(26)22(18(20)28)33-10(2)30)24(13)12-6-4-3-5-11(12)23(31)35-24/h3-8H,1-2H3

InChI key

ADWIDTFZSUABGT-UHFFFAOYSA-N

Application

Eosin diacetate is used as a fluorogenic probe to detect cellular esterase activity.

Other Notes

Fluorogenic substrate for esterases and lipases

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Flow cytometric analysis of cell cycle-dependent changes in cell thiol level by combining a new laser dye with Hoechst 33342.

M Poot et al.

Cytometry, 12(2), 184-187 (1991-01-01)

By halogenation of methylfluorescein-diacetate (MFDA) or eosin-diacetate, two new dyes for cellular thiol compatible with visible laser excitation have become available. These probes circumvent the use of an ultraviolet (UV)-excitation system as required by bimane-based dyes and allow combination with

Wright-Giemsa and nonspecific esterase staining of cells.

W Strober

Current protocols in cytometry, Appendix 3, Appendix 3D-Appendix 3D (2008-09-05)

This appendix provides two protocols for staining cells. The stains used in the Wright-Giemsa protocol, the Romanowsky stains, are a mixture of methylene blue (and other closely related thiazine dyes) and eosin. The staining protocol is a two-stage method that

G.G. Guilbault et al.

Analytical Biochemistry, 14, 28-28 (1966)

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