17490

Bromocresol Purple

for microscopy (Hist., Vit.), indicator (pH 5.2-6.8)

• Synonym(s): 5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form
• Empirical Formula (Hill Notation): C₂₁H₁₆Br₂O₅S
• CAS Number: 115-40-2
• Molecular Weight: 540.22
• Beilstein/REAXYS Number: 359618
• EC Number: 204-087-8
• MDL number: MFCD00011681
• UNSPSC Code: 12171500
• PubChem Substance ID: 57647609
• NACRES: NA.47

Properties

• grade: for microscopy (Hist., Vit.); indicator (pH 5.2-6.8)
• form: powder
• technique(s): titration: suitable
• pH: 5.2-6.8, yellow to purple
• mp: 240 °C (dec.) (lit.)
• solubility: hot ethanol: 0.1 g/10 mL
• εmax: ≥400 at 414-424 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4
• InChI: 1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
• InChI key: ABIUHPWEYMSGSR-UHFFFAOYSA-N

Description

General description

Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Application

Spectrophotometric determination of serum-albumin

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves