D9015

3,3′-Diaminobenzidine tetrahydrochloride hydrate

≥96% purity (TLC), powder

• Linear Formula: C₁₂H₁₄N₄ · 4HCl · xH₂O
• CAS Number: 868272-85-9
• EC Number: 231-018-9
• MDL number: MFCD08273058
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894241
• NACRES: NA.47

Properties

• Product Name: 3,3′-Diaminobenzidine tetrahydrochloride hydrate, ISOPAC^®
• Quality Level: 100
• assay: ≥96% (TLC)
• form: powder
• color: off-white to faint red, to beige
• mp: 280 °C
• solubility: water: 50 mg/mL, clear to slightly hazy
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
• InChI: 1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2
• InChI key: DXWSCXIZIHILNP-UHFFFAOYSA-N

Description

Application

3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridization.[1][2][3]

Biochem/physiol Actions

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.[4][5][6]

Packaging

Weight approximate. Packaged in a 100 mL serum bottle with silicon/PTFE septum and aluminum tear seal.

Caution

Injecting a compatible solvent permits preparation of any desired strength solution without exposure.

Reconstitution

Dissolving the contents in 100 mL of solvent yields a 0.1% solution.

Legal Information

Isopac is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H302,H319,H341,H350
• pcodes: P202 - P264 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)