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201308

Sigma-Aldrich

Pyronin B

certified by the Biological Stain Commission, Dye content 40 %

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About This Item

Empirical Formula (Hill Notation):
C42H54Cl8Fe2N4O2
CAS Number:
2150-48-3
Molecular Weight:
1042.22
Colour Index Number:
45010
Beilstein/REAXYS Number:
3846969
EC Number:
218-429-9
MDL number:
MFCD03701356
UNSPSC Code:
12171500
PubChem Substance ID:
24852004

grade

certified by the Biological Stain Commission

form

solid

composition

Dye content, 40%

mp

174-176 °C (lit.)

λmax

553 nm

SMILES string

[Cl-].Cl[Fe](Cl)Cl.CCN(CC)c1ccc2cc3ccc(cc3[o+]c2c1)N(CC)CC

InChI

1S/C21H27N2O.4ClH.Fe/c1-5-22(6-2)18-11-9-16-13-17-10-12-19(23(7-3)8-4)15-21(17)24-20(16)14-18;;;;;/h9-15H,5-8H2,1-4H3;4*1H;/q+1;;;;;+3/p-4

InChI key

SUVPQRYDMCNTPV-UHFFFAOYSA-J

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Suitability

Certified for use in the methyl green-pyronin method for differential coloration of nucleic acids.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
03001PD
06516TY
11302AO
0304HH

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Nuclear dyes and cytoplasmic staining.
G Diaz et al.
Cytometry, 29(4), 375-376 (1998-01-24)
Feng Gao et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 60(11), 2505-2509 (2004-08-06)
This is the first report on the determination of nucleic acids with Pyronine B (PB) sensitized by cetyltrimethylammonium bromide (CTMAB) with resonance light-scattering (RLS) technique. Under the experimental conditions (1 x 10(-5) mol l(-1) PB, 1 x 10(-5) mol l(-1)
C A Mannella et al.
Biochimica et biophysica acta, 981(2), 363-366 (1989-06-06)
Inhibition of mitochondrial respiration by hydrophobic fluorescent dyes (Rhodamine 6G, Safranine O, Pyronine B) is much less potentiated by digitonin-lysis of the outer membrane than that by polyamines or adriamycin. This situation may be explained by impermeability of the anion-selective
Behzad Rezaei et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 66(4-5), 869-873 (2006-07-29)
A new simple, selective, high sensitive and rapid method has been developed for spectrophotometric determination of ultra trace amounts of ruthenium based on its catalytic effect on the oxidation of pyronin B by periodate at lambdamax=555 nm. The described method
T Frey
Cytometry, 21(3), 265-274 (1995-11-01)
Apoptotic thymocytes were found to be much dimmer than normal thymocytes when stained with several nucleic acid dyes. These dyes provide a quick and simple assay for apoptosis which works for live cells and does not require a UV laser.
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