Recommended Products
usage
sufficient for 96 multiwell tests
manufacturer/tradename
Roche
shipped in
wet ice
storage temp.
2-8°C
General description
Detection limit: 0.28 μM for nitrate, and 0.32 μM for nitrite.
Linearity: The method is linear in the range from 0.28 μM to 80.0 μM nitrate and from 0.32 M to 108.0 μM nitrite.
Specificity
Under the given conditions, nitrate reductase reacts specifically with nitrate ions.
Application
Determination of nitric oxide (NO) via nitrite in biological fluids in a microplate. Nitrate present in the sample is reduced to nitrite by nitrate reductase.
Packaging
1 test combination containing 7 components.
Preparation Note
Working concentration: The standard material recommended is Na nitrate, but the concentrations given for the calibration curve are for the nitrate ion only. The reason for this is that it is not known which cation is present in a sample. To emphasize this, instructions indicate the conversion factor from NaNO2 to nitrite (NO2-).
Working solution: Preparation of the solutions
Storage conditions (working solution): Reaction mixture 2: Prepare immediately before use and bring to 15 to 25 °C before use
Color reagent I: sulfanilamide, stabilized, 8 ml
Potassium nitrate standard, aliquots: stable at -15 to -25 °C for 3 month
Working solution: Preparation of the solutions
- Use the contents of bottle 1 undiluted at 15 to 25 °C before use.
- Place three tablets from bottle 2 in a beaker and dissolve in 1 ml of buffer from bottle 1; use the tweezers provided to remove the tablets from the bottle. The resulting solution is reaction mixture 2 and is sufficient for about 20 incubation assays.
- Dissolve the contents of one bottle 3 in 0.6 ml of double-dist. water to give solution 3.
- Use the contents of bottle 4 undiluted.
- Use the contents of bottle 5 undiluted.
- Dilute the contents
Storage conditions (working solution): Reaction mixture 2: Prepare immediately before use and bring to 15 to 25 °C before use
Color reagent I: sulfanilamide, stabilized, 8 ml
Potassium nitrate standard, aliquots: stable at -15 to -25 °C for 3 month
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Potassium Phosphate Buffer, pH 7.5
- Coenzyme
- Nitrate Reductase (2)
- Color Reagent I, consisting of sulfanilamide
- Color Reagent II, consisting of N-(1-naphthyl)ethylendiamine dihydrochloride
- Potassium nitrate standard 80.0 mM
- Microtiter plate, a frame with 12 modules 8 wells each
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
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