11585541001

PCR Core Kit^PLUS

sufficient for ≤50 reactions, optimum pH ~9.0 (20 °C), optimum reaction temp. 72 °C

• Synonym(s): PCR
• UNSPSC Code: 41106300

Properties

• usage: sufficient for ≤50 reactions
• manufacturer/tradename: Roche
• parameter: 72 °C optimum reaction temp.
• optimum pH: ~9.0 (20 °C)
• storage temp.: −20°C

Description

General description

The PCR Core Kit^PLUS supplies all basic reagents to perform a polymerase chain reaction with the exception of template DNA and appropriate primers. The kit uses Taq DNA Polymerase as the polymerase. In addition UNG, heat-labile is supplied in the kit for efficient degradation of contaminating DNA from previous PCR amplifications.

Application

The PCR Core Kit^PLUS is the solution for laboratories that have to run PCRs with non-standard reaction conditions and also have to make sure that samples are not contaminated with DNA from earlier amplification reactions.

Features and Benefits

The PCR Core KitPLUS is from Thermus aquaticus BM , expressed in E. coli . This highly processive 5′ → 3′ DNA polymerase lacks 3′ → 5′ exonuclease activity. It is stable during prolonged elevated temperatures (+95°C). During PCR, it retains over 80% activity after 30 cycles (1 minute, +95°C; 1 minute, +37°C; 1 minute, +72°C). The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 95kD.

• Reliable reproducible results:

High lot-to-lot consistency.

• Flexible:

Quickly set up the appropriate PCR conditions.

• Optimize Mg²⁺:

The buffers is supplied without MgCl₂.

• Eliminate false-positive results:

Remove carryover contamination using Uracil-DNA Glycosylase.

Packaging

1 kit containing 6 components.

Quality

Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.

Specifications

The PCR Core Kit^PLUS can reduce the risk of false-positive results by perventing PCR carryover contamination. Supplied Uracil-DNA Glycosylase (UNG) catalyzes the removal of uracil from single- and double-stranded DNA originating from the incorporation of dUTP during DNA replication or deamination of cytosine. The abasic sites are susceptible to cleavage by heat under alkaline conditions. Ribouracil residues in RNA and dUTP are not substrates for UNG. Using this kit can eliminate preexisting PCR products by at least a factor of 10⁷.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

Kit Components Only

• Uracil-DNA Glycosylase, heat-labile 1 U/μl
• Taq DNA Polymerase 5 U/μl
• dNTP Stock Solution (10 mM of each dNTP)
• PCR Buffer with MgCl₂ 10x concentrated
• PCR Buffer without MgCl₂ 10x concentrated
• MgCl₂ Stock Solution 25 mM

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: does not flash
• flash_point_c: does not flash