11373099910
Roche
RNA Molecular Weight Marker III, DIG-labeled
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About This Item
UNSPSC Code:
41105335
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form
solution
packaging
pkg of 200 μL (2 μg)
manufacturer/tradename
Roche
concentration
10 ng/μL
shipped in
dry ice
storage temp.
−20°C
General description
The fragments are prepared by in vitro transcription of linearized plasmids with SP6 or T7 RNA Polymerase in separate reactions. The transcripts are then combined at a ratio that gives bands of uniform intensity when separated by gel electrophoresis.
The transcripts are labeled in a photodigoxigenin reaction so that a digoxigenin moiety is present every 200th to 300th nucleotide.
Size Range: 0.3 to 1.5 kb
The transcripts are labeled in a photodigoxigenin reaction so that a digoxigenin moiety is present every 200th to 300th nucleotide.
Size Range: 0.3 to 1.5 kb
Application
Sequence
Five fragments: 310, 438, 575, 1049, and 1517 bases.
Preparation Note
Working concentration: Transfer 40 to 100 ng of labeled RNA Molecular Weight Marker I per lane or 20 to 50 ng of RNA Molecular Weight Marker II or III per lane, depending on the reaction time in the detection step, to produce clear banding patterns.
Note: Taking the lane (slot) size of the agarose gel into account, load 100 ng for RNA marker I and 50 ng for RNA marker II or III.
Note: Taking the lane (slot) size of the agarose gel into account, load 100 ng for RNA marker I and 50 ng for RNA marker II or III.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
No data available
flash_point_c
No data available
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