Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

11348639001

Sigma-Aldrich

Propidium iodide

96% (TLC), solution, 0.5 mg/mL, suitable for hematology, histology

Sign Into View Organizational & Contract Pricing

Select a Size

All Photos(1)

Select a Size

Change View

About This Item

UNSPSC Code:
12171500

assay

96% (TLC)

form

solution

packaging

pkg of 20 mL (0.5mg/mL)

manufacturer/tradename

Roche

concentration

0.5 mg/mL

pH

7.0

εmax

6000 at 493 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

General description

Propidium iodide is a fluorogenic compound. It is used in nuclear staining as it binds to nucleic acids stoichiometrically.[1]
Stain for non-viable cells or DNA counterstain in flow cytometric studies.
Emission maximum: 632nm
Excitation maximum: 493nm

Application

Propidium iodide is used for:
  • Discrimination of viable and non-viable cells in flow cytometry
  • Determination of DNA content in flow cytometry[2][3][4]

Physical form

Solution, red/orange, containing 0.5mg/mL propidium iodide in phosphate-buffered saline (pH approx. 7.4) with 0.09% sodium azide.

Preparation Note

Working concentration: 0.5mg/mL for flow cytometry

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
26276400
32701100
20799200
11049100
10164200

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Carlo Riccardi et al.
Nature protocols, 1(3), 1458-1461 (2007-04-05)
Since its introduction, the propidium iodide (PI) flow cytometric assay has been widely used for the evaluation of apoptosis in different experimental models. It is based on the principle that apoptotic cells, among other typical features, are characterized by DNA
Baeck-seung Lee et al.
Molecular and cellular biology, 33(9), 1768-1781 (2013-02-27)
Recombination-activating gene 1 protein (RAG1) and RAG2 are critical enzymes for initiating variable-diversity-joining (VDJ) segment recombination, an essential process for antigen receptor expression and lymphocyte development. The transcription factor BCL11A is required for B cell development, but its molecular function(s)
Zhenyi An et al.
Autophagy, 10(10), 1702-1711 (2014-08-16)
In response to starvation, cells undergo increased levels of autophagy and cell cycle arrest but the role of autophagy in starvation-induced cell cycle arrest is not fully understood. Here we show that autophagy genes regulate cell cycle arrest in the
Marina G Gobbo et al.
Journal of diabetes research, 2015, 538529-538529 (2015-08-22)
The antitumor properties of melatonin (MLT) are known for prostate cancer cells. This study investigated whether MLT affects prostate maturation and interferes with tissue injuries induced by diabetes. MLT was administered to Wistar rats from 5 weeks of age in

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service