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usage
sufficient for 25 blots (10 cm x 10 cm each)
manufacturer/tradename
Roche
greener alternative product characteristics
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shipped in
wet ice
storage temp.
2-8°C
General description
Characterize carbohydrate moieties of glycoproteins or other, correspondingly fixed, glycoconjugates.
Application
The DIG Glycan Differentiation Kit is used to analyze carbohydrate structures by the specific binding of selected lectins. In addition to the differentiation of glycoproteins bound to nitrocellulose membranes, the lectins are also suited for the identification of carbohydrate structures on tissue sections.[1][2]
Packaging
1 kit containing 13 components.
Quality
Function tests: With the lectins GNA, SNA, MAA, and DSA 0.1 μg, and with the lectin PNA 1 μg of the control glycoproteins are detectable after a 1-min incubation in the staining solution.
Principle
Detection of digoxigenin-labeled lectins bound to specific carbohydrate structures on blots. Detection is performed using anti-digoxigenin alkaline-phosphatase conjugates. The digoxigenin-labeled lectins enable differentiation between complex and high-mannose chains, differentiation between a(2,3) and a(2,6) linkage of terminal sialic acids, and identification of the core disaccharide Galß(1,3)GalNAc of O-glycans.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Lectin GNA, digoxigenin-labeled
- Lectin SNA, digoxigenin-labeled
- Lectin MAA, digoxigenin-labeled
- Lectin PNA, digoxigenin-labeled
- Lectin DSA, digoxigenin-labeled
- Anti-digoxigenin-AP
- NBT/BCIP Solution
- Carboxypeptidase Y, Control Glycoprotein
- Transferrin, Control Glycoprotein, HIV tested
- Fetuin, Control Glycoprotein
- Asialofetuin, Control Glycoprotein
- Blocking Reagent
- Ponceau S solution
See All (13)
signalword
Danger
Hazard Classifications
Acute Tox. 3 - Acute Tox. 4 - Acute Tox. 4 Oral - Aquatic Chronic 2 Inhalation - Resp. Sens. 1 Dermal - Skin Sens. 1
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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Lifei Yang et al.
Journal of virology, 86(14), 7662-7676 (2012-05-04)
The development of a successful vaccine against human immunodeficiency virus type 1 (HIV-1) likely requires immunogens that elicit both broadly neutralizing antibodies against envelope spikes and T cell responses that recognize multiple viral proteins. HIV-1 virus-like particles (VLP), because they
Pablo Manfredi et al.
Molecular microbiology, 81(4), 1050-1060 (2011-07-19)
Capnocytophaga canimorsus are commensal Gram-negative bacteria from dog's mouth that cause rare but dramatic septicaemia in humans. C. canimorsus have the unusual property to feed on cultured mammalian cells, including phagocytes, by harvesting the glycan moiety of cellular glycoproteins. To
Monica Lopes-Ferreira et al.
Toxins, 14(1) (2022-01-21)
TmC4-47.2 is a toxin with myotoxic activity found in the venom of Thalassophryne maculosa, a venomous fish commonly found in Latin America whose envenomation produces an injury characterized by delayed neutrophil migration, production of major pro-inflammatory cytokines, and necrosis at
Francesco Renzi et al.
mBio, 6(2), e02507-e02507 (2015-03-05)
Capnocytophaga canimorsus is known to form two kinds of cells on blood agar plates (coccoid and bacillary), evoking phase variation. When grown in coculture with animal cells these bacteria appeared only as bacilli, but in the presence of vancomycin they
Frédéric Lauber et al.
mBio, 7(5) (2016-11-01)
Bacteria of the phylum Bacteroidetes, including commensal organisms and opportunistic pathogens, harbor abundant surface-exposed multiprotein membrane complexes (Sus-like systems) involved in carbohydrate acquisition. These complexes have been mostly linked to commensalism, and in some instances, they have also been shown
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