11102982001
Roche
Mro I (Acc III)
from Micrococcus roseus
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About This Item
UNSPSC Code:
41105600
biological source
bacterial (Micrococcus roseus)
form
solution
packaging
pkg of 100 U (1 - 5 U/μl)
manufacturer/tradename
Roche
parameter
37 °C optimum reaction temp.
shipped in
dry ice
storage temp.
−20°C
General description
Restiction endonuclease Mro I (Acc III) recognizes the sequence TCCGGA.[1]
Specificity
Recognition sites: T↓*CCGG°A
T*CCGG°A
Restriction site: T↓*CCGG°A
T↓*CCGG°A
Heat inactivation: There is no information available whether or not Mvn I can be heat inactivated.
T*CCGG°A
Restriction site: T↓*CCGG°A
T↓*CCGG°A
Heat inactivation: There is no information available whether or not Mvn I can be heat inactivated.
DNA Profile
Number of cleavage sites on different DNAs
- λ: 24
- φX174: 0
- Ad2: 8
- M13mp7: 0
- pBR322: 1
- pBR328: 1
- pUC18: 0
- SV40: 0
Unit Definition
One unit is the enzyme activity that completely cleaves 1 μg λDNA in one hour at +37 °C in a total volume of 25 μl (1x) SuRE/Cut Buffer A.
Analysis Note
SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity
The buffer in bold is recommended for optimal activity
- A: 100%
- B: 0-10%
- H: 0-10%
- L: 50-75%
- M: 50-75%
Activity in PCR buffer: Not tested
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Enzyme Solution
- SuRE/Cut Buffer A 10x concentrated
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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K Inagaki et al.
Bioscience, biotechnology, and biochemistry, 57(10), 1716-1721 (1993-10-01)
A restriction endonuclease, Aor13HI, an isoschizomer of BspMII, was purified to homogeneity from cell extracts of Acidiphilium organovorum strain 13H. The enzyme has a molecular mass of 60,000 daltons and consists of two subunits identical in molecular mass of 30,000
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