10602442001
Roche
α2-Macroglobulin
from bovine plasma
Synonym(s):
universal protease inhibitor
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About This Item
UNSPSC Code:
12352204
Recommended Products
biological source
bovine plasma
form
lyophilized (Contains 6% BSA)
packaging
pkg of 25 IU
manufacturer/tradename
Roche
technique(s)
blocking: suitable
pH range
4-7
UniProt accession no.
shipped in
wet ice
Gene Information
bovine ... A2M(513856)
General description
α2-Macroglobulin belongs to the macroglobulin superfamily. It is a high molecular weight tetrameric glycoprotein with receptor-binding domain (RBD), thioester-containing domain (TED) domain and C1r/C1s, Uegf, Bmp1 (CUB) domain.[1]
application
α2-Macroglobulin has been used as a component of homogenization buffer for processing hemibrains of young mice.[2]
Biochem/physiol Actions
α2-Macroglobulin (α2M) binds several hormones and may regulate their activity. α2M also aids protection against various infections.[1]
Sequence
α2-macroglobulin, a glycoprotein, is a tetramer of identical subunits, which are Iinked in pairs by disulfide bonds.
Preparation Note
Working solution: Recommended solvent is distilled water.
Storage conditions (working solution): -15 to -25 °C
Storage conditions (working solution): -15 to -25 °C
Reconstitution
Soluble in water. Stable for at least one week at room temperature (15 to 25 °C), or 3 weeks at 2 to 8 °C. Can also be frozen in aliquots at -15 to -25 °C, where it remains stable for at least 6 months. Sensitive to acidic pH, denatured below pH 4.0. Ammonia methylamine and hydroxylamine (above pH 7.0) cause irreversible conversion to the inactive form.
Note: Do not use α2-macroglobulin in the presence of DTT since it causes a (reversible) dissociation into inactive subunits. α2-macroglobulin acts by physically entrapping endoproteinases, usually in a 1:1 ratio.
Note: Do not use α2-macroglobulin in the presence of DTT since it causes a (reversible) dissociation into inactive subunits. α2-macroglobulin acts by physically entrapping endoproteinases, usually in a 1:1 ratio.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)
Analysis Note
Specific activity: approximately 1 inhibitor U/mg protein (tested with trypsin at +25°C and Chromozym TRY as substrate).
Crossreactivity with pregnancy associated glycoproteins: deglycosylation of α2-macroglobulin is recommended.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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Journal of cellular physiology, 228(8), 1665-1675 (2012-10-23)
Alpha macroglobulins are large glycoproteins which are present in the body fluids of both invertebrates and vertebrates. Alpha-2-macroglobulin (α2 M), a key member of alpha macroglobulin superfamily, is a high-molecular weight homotetrameric glycoprotein. α2 M has many diversified and complex
A J Barrett et al.
The Biochemical journal, 181(2), 401-418 (1979-08-01)
alpha 2-Macroglobulin (alpha 2M) was isolated from human plasma by a four-step procedure: poly(ethylene glyco) fractionation, gel chromatography, euglobulin precipitation and immunoadsorption. No contaminants were detected in the final preparations by electrophoresis or immunoprecipitation. The protein ran as a single
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