10280879001

5-Bromo-2′-deoxyuridine

>97%, crystalline solid, pkg of 1 g

• Synonym(s): 5-Bromo-2′-deoxyuridine, brdu, 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
• Empirical Formula (Hill Notation): C₉H₁₁BrN₂O₅
• CAS Number: 59-14-3
• Molecular Weight: 307.10
• Beilstein/REAXYS Number: 30395
• MDL number: MFCD00006529
• UNSPSC Code: 12352204
• PubChem Substance ID: 329749004

Properties

• Quality Level: 100
• assay: >97%
• form: crystalline solid
• mol wt: 307.1
• packaging: pkg of 1 g
• manufacturer/tradename: Roche
• mp: 191-194 °C (dec.) (lit.)
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
• InChI key: WOVKYSAHUYNSMH-RRKCRQDMSA-N

Description

General description

It is conventionally used as a marker for S-phase synthesis of DNA.

Application

5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.

Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.

Biochem/physiol Actions

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

Quality

Purity: 97% (from N), chromatographically homogeneous

Preparation Note

Working concentration: According to literature for cell culture, a final concentration of 10 μmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.

Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H340,H361fd
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 1B - Repr. 2
• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable