10223581001
Roche
Poly(dA)
Poly-deoxy-adenylic acid
Synonym(s):
PCR
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About This Item
UNSPSC Code:
41105600
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form
lyophilized
mol wt
330 Da
packaging
pkg of 5 U
manufacturer/tradename
Roche
absorbance ratio
A290/260 nm 0.03-0.12
A280/260 nm 0.28-0.39
A250/260 nm 0.84-0.94
storage temp.
−20°C (−15°C to −25°C)
General description
Poly(dA) is a polymer of polydeoxyadenylic acid and takes up a single stranded structure. It is susceptible to UV irradiation resulting in photoreaction due to the presence of adjacent deoxyadenylic acid units. Poly(dA) along with β-glucan is used for the conjugation and delivery of DNA into cell lines.
Application
Poly(dA) is suitable for use in:
- DNA polymerase assay and as a buffer component for the dilution of protein DNA complex for electrophoretic mobility shift assay (EMSA) studies
- as ssDNA substrate for DNA helicase gene 40 product (G40P) NTP hydrolysis
- in Pyrococcus abyssi DNA polymerase assay
Sequence
Chain Length Mean chain length lot 20-17: 250-500 bases.
Unit Definition
1 A260 unit corresponds to 40 μg ssDNA.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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