10105678001
Roche
Glutathione Reductase (GR)
from yeast
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About This Item
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biological source
yeast
form
suspension
specific activity
~120 units/mg protein (At 25 °C with oxidized glutathione as the substrate; standardized with BSA.)
packaging
pkg of 1 mL (5 mg)
manufacturer/tradename
Roche
storage temp.
2-8°C
General description
Glutathione:NADP+ oxidoreductase
Application
Glutathione reductase (GR) has been used for the reduction of GSSG (glutathione disulfide) to GSH (glutathione).[1]
Biochem/physiol Actions
Glutathione Reductase (GR) is a NADPH-dependent flavoprotein that catalyzes the reduction of glutathione disulfide (GSSG) to glutathione (GSH). It is involved in the maintenance of glutathione levels, which is important for oxidation–reduction processes, detoxication and several other cellular functions.[2] In yeast, GR protects the cells from oxidative stress and is transcriptionally regulated by yAP-1 (yes-associated protein 1) protein.[3]
Quality
Contaminants: <0.01% NADPH oxidase and 6-PGDH each, <0.1% G6P-DH
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
The ammonium sulfate suspension is stable at 2 to 8 °C until the expiration date printed on the label. It may be used directly in assays for glutathione.
Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Other Notes
For life science research only. Not for use in diagnostic procedures.
related product
Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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C M Grant et al.
Molecular microbiology, 21(1), 171-179 (1996-07-01)
Glutathione (GSH) is an abundant cellular thiol which has been implicated in many cellular processes including protection against xenobiotics, carcinogens and free radicals. Utilization of GSH in both enzymic and non-enzymic defence mechanisms results in its conversion to the oxidized
Glutathione reductase.
I Carlberg et al.
Methods in enzymology, 113, 484-490 (1985-01-01)
Trent E Tipple et al.
Methods in molecular biology (Clifton, N.J.), 889, 315-324 (2012-06-07)
We present two different methods for determining levels of glutathione in complex biological samples and plasma. The DTNB/GR enzyme recycling method is sensitive and requires no specialized equipment. The HPLC method is particularly useful for situations in which sample amounts
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