F2502

Fluorescein isothiocyanate isomer I

90%

• Synonym(s): FITC, Fluorescein 5-isothiocyanate
• Empirical Formula (Hill Notation): C₂₁H₁₁NO₅S
• CAS Number: 3326-32-7
• Molecular Weight: 389.38
• Beilstein/REAXYS Number: 1407295
• EC Number: 222-042-0
• MDL number: MFCD00005063
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894823

Properties

• assay: 90%
• form: powder
• mp: >360 °C (lit.)
• solubility: acetone: 1 mg/mL
• λmax: 499 nm
• fluorescence: λ_ex 492 nm; λ_em 518 nm (green)
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

Application

Fluorescent labeling reagent for proteins.[1][2][3][4][5] Used in the fluorescent antibody technique for rapid identification of pathogens.[6][7]

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Other Notes

This product has been replaced by F7250-SIGMA | Fluorescein isothiocyanate isomer I suitable for protein labeling, ≥90% (HPLC), powder

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves