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858811

Sigma-Aldrich

5-Bromo-2′-deoxyuridine

97%

Synonym(s):

5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR

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About This Item

Empirical Formula (Hill Notation):
C9H11BrN2O5
CAS Number:
59-14-3
Molecular Weight:
307.10
Beilstein/REAXYS Number:
30395
EC Number:
200-415-9
MDL number:
MFCD00006529
UNSPSC Code:
41106305
PubChem Substance ID:
24888474

assay

97%

optical activity

[α]22/D +23.0°, c = 1 in H2O

mp

191-194 °C (dec.) (lit.)

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O

InChI

1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1

InChI key

WOVKYSAHUYNSMH-RRKCRQDMSA-N

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Biochem/physiol Actions

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

replaced by

Product No.
Description
Pricing

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Muta. 1B - Repr. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 2

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
02731MH
08713JH
08313JH
12722CC
12622CC

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the stability of Product 858811, 5-Bromo-2´-deoxyuridine (BrdU)?

    The comparable Sigma product, B5002, has a recommended retest specification of 2 years. We have observed by TLC that the material is unchanged after 3 years. Solutions at neutral pH should be stable if stored frozen for up to 6 months.

  5. What can I use to detect BrdU, 5-Bromo-2´-deoxyuridine, in vivo and in vitro?

    For the immunohistochemical detection of BrdU, we recommend the monoclonal anti-BrdU antibody, Product No. B2531.

  6. What are the hazards associated with Product 858811, 5-Bromo-2´-deoxyuridine (BrdU)?

    BrdU is a known carcinogen, and caution should be exercised when handling. Please refer to the MSDS.

  7. How long do I treat cells with Product 858811, 5-Bromo-2´-deoxyuridine (BrdU) to incorporate it into the DNA?

    Actively growing cells may be treated with 10 μM BrdU for 1 hour.

  8. How does Product 858811, 5-Bromo-2´-deoxyuridine (BrdU), work as a thymidine analog?

    BrdU replaces thymidine during the S-phase of cell division.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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