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224030

Sigma-Aldrich

CHES

Synonym(s):

2-(Cyclohexylamino)ethanesulfonic acid

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About This Item

Empirical Formula (Hill Notation):
C8H17NO3S
CAS Number:
Molecular Weight:
207.29
Beilstein/REAXYS Number:
2967601
MDL number:

pH

8.6-10.0

useful pH range

8.6-10.0

pKa (25 °C)

9.3

SMILES string

OS(=O)(=O)CCNC1CCCCC1

InChI

1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)

InChI key

MKWKNSIESPFAQN-UHFFFAOYSA-N

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General description

2-(cyclohexylamino)ethanesulfonic acid (CHES) is a zwitterionic amphiphilic compound, which consists of a hydrophobic cyclohexyl, a hydrophilic amino, and a sulfonic acid group. It is used as a buffer in specific biological processes; the useful pH range being 8.6-10.

Application

CHES is used as a buffer for the investigation of pH-dependent processes in a biological research application such as enzymology. It is also used as a refolding buffer for the separation of chiral polychlorinated biphenyls by micellar electrokinetic chromatography. Bovine whey proteins were detected using CHES buffer of pH=10.2.

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DFT analysis on the molecular structure, vibrational and electronic spectra of 2-(cyclohexylamino) ethanesulfonic acid
Devi TSR, et al.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 137, 761-777 (2015)

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