216720

4′,5′-Dibromofluorescein

Dye content 95 %

• Synonym(s): Solvent Red 72
• Empirical Formula (Hill Notation): C₂₀H₁₀Br₂O₅
• CAS Number: 596-03-2
• Molecular Weight: 490.10
• Colour Index Number: 45370:1
• Beilstein/REAXYS Number: 57189
• EC Number: 209-876-0
• MDL number: MFCD00005042
• UNSPSC Code: 12171500
• PubChem Substance ID: 24852976
• NACRES: NA.47

Properties

• assay: >95% (TLC)
• form: powder
• composition: Dye content, 95%
• mp: 270-273 °C (lit.)
• λmax: 450 nm
• ε (extinction coefficient): ≥5000 at 445-451 nm; ≥50000 at 227-233 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Oc1ccc2c(Oc3c(Br)c(O)ccc3C24OC(=O)c5ccccc45)c1Br
• InChI: 1S/C20H10Br2O5/c21-15-13(23)7-5-11-17(15)26-18-12(6-8-14(24)16(18)22)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
• InChI key: ZDTNHRWWURISAA-UHFFFAOYSA-N

Description

General description

4′,5′-Dibromofluorescein (DBF) is a fluorone dye. Fluorone dyes are hydroxyl-xanthene dyes (homologues of fluorescein) and have been the most widely used class of organic fluorophores for labeling and sensing biomolecules. DBF is routinely used as a ligand for studying the properties of protein using spectral analysis.

Application

4′,5′-Dibromofluorescein has been used to stain proteins in 1-D and 2-D sodium dodecyl sulfatepolyacrylamide gel electrophoresis SDS-PAGE. This method is rapid, economical, and highly sensitive in comparison with other proteomic research staining methods.

Safety Information

• pictograms: GHS06
• signalword: Danger
• hcodes: H301
• pcodes: P301 + P310
• Hazard Classifications: Acute Tox. 3 Oral
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges