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D3385

Sigma-Aldrich

3′-Dephosphocoenzyme A

≥90% (HPLC)

Synonym(s):

3′-Desphosphocoenzyme A, dpCoA

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5 MG
$225.00
25 MG
$807.00

$225.00

Estimated to ship onMarch 29, 2025

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5 MG
$225.00
25 MG
$807.00

About This Item

Empirical Formula (Hill Notation):
C21H35N7O13P2S
CAS Number:
3633-59-8
Molecular Weight:
687.55
MDL number:
MFCD00057010
UNSPSC Code:
41106305
PubChem Substance ID:
24893748
NACRES:
NA.51

$225.00

Estimated to ship onMarch 29, 2025

biological source

synthetic (organic)

Quality Level

200

assay

≥90% (HPLC)

form

powder

impurities

≤1% CoA

solubility

H2O: soluble-50 mg/mL, clear to slightly hazy, colorless to faintly yellow

storage temp.

−20°C

SMILES string

CC(C)(COP(O)(=O)OP(O)(=O)OCC1OC(C(O)C1O)n2cnc3c(N)ncnc23)C(O)C(=O)NCCC(=O)NCCS

InChI

1S/C21H35N7O13P2S/c1-21(2,16(32)19(33)24-4-3-12(29)23-5-6-44)8-39-43(36,37)41-42(34,35)38-7-11-14(30)15(31)20(40-11)28-10-27-13-17(22)25-9-26-18(13)28/h9-11,14-16,20,30-32,44H,3-8H2,1-2H3,(H,23,29)(H,24,33)(H,34,35)(H,36,37)(H2,22,25,26)

InChI key

KDTSHFARGAKYJN-UHFFFAOYSA-N

Application

3′-Dephosphocoenzyme A has been used as a substrate to initiate transcription.[1][2][3]
Dephosphocoenzyme A is the direct precursor of coenzyme A. It is used for the synthesis of coenzyme A by bifunctional CoA Synthase (CoASy, 4′-phosphopantetheine adenylyltransferase/dephospho-CoA kinase) which performs the transfer of the γ-phosphate of ATP to dephosphocoenzyme A.

Biochem/physiol Actions

3′-Dephosphocoenzyme A (DepCoA), also called desphosphoCoA (dpCoA), is synthesized from 4-phosphopantetheine by the enzyme phosphopanthetheine adenylyltransferase. It is an intermediate in coenzyme A biosynthetic pathway. In the presence of dephospho-CoA kinase (CoaE), dephosphocoenzyme A is converted to coenzyme A.[4] 3′-Dephosphocoenzyme shares adenosine-diphosphate substructure as like the purines and is a non-canonical nucleotide and is an initiator of transcription initiation.[5] It is routinely used in in vitro transcription studies to initiate RNA synthesis.[6]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000382424
0000360427
0000382424
0000360427
0000253943

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Efficient one-pot enzymatic synthesis of dephospho coenzyme A
Sapkota K and Huang F
Bioorganic Chemistry, 76, 23-27 (2018)
Azusa Seto et al.
Proteins, 58(1), 235-242 (2004-11-05)
Dephosphocoenzyme A kinase (DCK) catalyzes phosphorylation in the final step of coenzyme A (CoA) biosynthesis. In this phosphorylation process, domain movements play a very important role. To reveal the structural changes induced by ligand binding, we determined the crystal structure
RNA capping by transcription initiation with non-canonical initiating nucleotides (NCINs): determination of relative efficiencies of transcription initiation with NCINs and NTPs
Bird JG, et al.
Bio-protocol, 7(12) (2017)
Ishac Nazi et al.
Analytical biochemistry, 324(1), 100-105 (2003-12-05)
A rapid and stoichiometric method for the synthesis of analogues of coenzyme A is described. The method links the enzymes pantothenate kinase, phosphopantotheine adenylyltransferase, and dephosphocoenzyme A kinase in vitro to generate a variety of CoA analogues from chemically synthesized
S Hoenke et al.
Biochemistry, 39(43), 13223-13232 (2000-10-29)
Malonate decarboxylase from Klebsiella pneumoniae consists of four subunits MdcA, D, E, and C and catalyzes the cleavage of malonate to acetate and CO(2). The smallest subunit MdcC is an acyl carrier protein to which acetyl and malonyl thioester residues
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